Protein_Domain
Part:BBa_K112610:Design
Designed by: Sherine Cheung Group: iGEM08_UC_Berkeley (2008-10-22)
{PhoA pp>} in BBb
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
N/A
Source
Construction of {PhoA>} basic part K112610 PCR cb10007/sc013 on MG1655 (94 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112610 ------------------------------------------------------ cb10007 Fwd biobricking of {prepro>} cgataGAATTCatgAGATCTatgaaacaaagcactattgcactggc sc013 Reverse biobricking of {prepro>} ccagtGGATCCggcttttgtcacaggggtaaac